Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions

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Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions.

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used to obtain high resolution separation of complex mixtures of proteins. The method initially denatures the proteins that will undergo electrophoresis. Although covalent structural features of resolved proteins can be determined with SDS-PAGE, functional properties are destroyed, including the presence of non-cov...

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An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...

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ژورنال

عنوان ژورنال: Metallomics

سال: 2014

ISSN: 1756-5901,1756-591X

DOI: 10.1039/c4mt00033a